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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in several tumors, which leads to chromosome instability, and therefore might contribute to tumorigenesis. This protein was uncovered to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital position in advertising mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been explained. Two additional splice variants ended up claimed but in depth nucleotide sequence information and facts is not nonetheless available.
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Blocking peptides are peptides that bind specifically to your goal antibody and block antibody binding. These peptide usually contains the epitope identified from the antibody.
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This guidebook outlines more doable optimization of cellular and immunoassay parameters to guarantee the best possible benefits are...
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This information outlines additional possible optimization of mobile and immunoassay parameters to make certain the absolute best final results are...
Antibodies bound to the blocking peptide not bind into the epitope about the target protein. By evaluating the staining from the blocked antibody as opposed to the antibody alone, one can see which staining is unique.
Target Facts Cyclin E belongs towards the highly conserved cyclin loved ones, whose customers are characterised by a extraordinary periodicity in protein abundance through the cell cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation designs which contribute on the temporal coordination of every mitotic party. Cyclin E forms a posh with and capabilities as being a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S changeover. This protein accumulates at the G1-S phase boundary and it is degraded as cells progress by means of S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...
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